DNA Nanostructures as Models for Evaluating the Role of Enthalpy and Entropy in Polyvalent Binding

Authors: Nangreave, J., Yan, H., and Liu, Y.
Title: DNA Nanostructures as Models for Evaluating the Role of Enthalpy and Entropy in Polyvalent Binding
Source: Journal of the American Chemical Society
Year: 2011
Volume: 133
Pages: 4490-4497

ABSTRACT:

DNA nanotechnology allows the design and construction of nanoscale objects that have finely tuned dimensions, orientation, and structure with remarkable ease and convenience. Synthetic DNA nanostructures can be precisely engineered to model a variety of molecules and systems, providing the opportunity to probe very subtle biophysical phenomena. In this study, several such synthetic DNA nanostructures were designed to serve as models to study the binding behavior of polyvalent molecules and gain insight into how small changes to the ligand/receptor scaffolds, intended to vary their conformational flexibility, will affect their association equilibrium. This approach has yielded a quantitative identification of the roles of enthalpy and entropy in the affinity of polyvalent DNA nanostructure interactions, which exhibit an intriguing compensating effect.


Date of online publication: Mon, 2011-03-07
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